Query Information

ORIGIN plant
BEGIN
>MYSEQ
 YPWKNSVFKV ILTRNTRSRF WILLELQEIR RCAKCNGVTT VKMKQLGKEK
 KSFAIFPTFS LDLLKSRGRD YFGGDLHPLC NLLEIIPIEK FLLMWIPLCI
 ILCEHHIQKQ IINKRIYAIK IMHHAGYCVC GVYFVTIQKN IIYKIKRIHY
 LDENLRVKGD FRNMEKRNKG YKITTYMLYF DWYFLFHNIN GTGTRFEFKS
 EFKMEFENKE KENIKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          218 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 85 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 92   Charge diffirence(C-N):  3.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:   1.35
     Net Charge of CR: 2
     Discriminant Score:     -6.21
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.58
     Possible cleavage site: 18
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -3.82  threshold: -2.0
     INTEGRAL    Likelihood = -3.82   Transmembrane   85 - 101 (  85 - 104)
     PERIPHERAL  Likelihood =  2.28
     modified ALOM score:   0.86
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 102 to 218 (118 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 21
     SRFWIL
Discrimination of mitochondrial target seq.: 
     positive ( 2.45)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.68 at (52)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  2.77
Chloroplast protein?  Status: negative (-9.80)

*** Reasoning Step: 2

> Relative position of the end of the tail: 46%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 21)  from: 53  to: 56  Score:  4.0
Mitochondrial inner membrane?  Score:  0.52
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.173
SKL motif (signal for peroxisomal protein): 
     pos: -1(218), count: 0   
Amino Acid Composition Tendency for Peroxisome:   4.55
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score:  1.34  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 214 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.10)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.685(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.345(Affirmative) < succ>


----- The End -----