Query Information

ORIGIN plant
BEGIN
>MYSEQ
 YFGWEQYWEK YKLWRTVYAW YCISHEFKWQ LWWGYQHSRI QPYWYASTSI
 PTIYELAWKF LPNTWRVTIS ESSTVRKWFF RIFWNAIRKL RQCSICFDPS
 IWDTVHKDNF VLHYEAVEKR PVLPFAGQNI RRELGSPAFE VNLVSVAVVM
 DGLSSYKMIL ELMTPRWQLK CSDLDFLHNL LALFSRAIVV PMLLDLLLLL
 LRSHRDDNKC FDRYESAKKK KK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          222 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 134 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 141   Charge diffirence(C-N): -1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   1
     Peak Value of UR:   1.10
     Net Charge of CR: -1
     Discriminant Score:    -10.88
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.04
     Possible cleavage site: 24
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -5.15  threshold: -2.0
     INTEGRAL    Likelihood = -5.15   Transmembrane  185 - 201 ( 180 - 204)
     PERIPHERAL  Likelihood =  0.32
     modified ALOM score:   1.13
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 184 (184 Residues)
( 4) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 4
     Uncleavable? Ipos set to: 14
Discrimination of mitochondrial target seq.: 
     negative (-2.82)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.40 at (59)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region: -0.90
Chloroplast protein?  Status: negative (-12.70)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 82%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.226
SKL motif (signal for peroxisomal protein): 
     pos: -1(222), count: 0   
Amino Acid Composition Tendency for Peroxisome:  11.64
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -6.24  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 217 (5) KKKK
     Found: pos: 218 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:  75 (3) RK WFFRIFWNAI RKLRQ
Final Robbins Score (nucleus):  0.60
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.50)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.850(Affirmative) < succ>
                          nucleus --- Certainty= 0.800(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.440(Affirmative) < succ>


----- The End -----