Query Information

ORIGIN plant
BEGIN
>MYSEQ
 YCKAVLITIL FCKERAHDEP RIDSPLHALS PPSSPSAASY SSKFQRNKET
 SNQNPKEHDS TPAVRSVYEP TNISSLLLLQ SQVPVHFTCF ACACRSERYG
 SVKSLAEVTT ASALLEATPR HADRCARRRP PSCSDRSQLV VVAKPPQLAC
 EPDVSLLLLL VVFGRRVGGR SGRRRAVRGR RRRHQLLPKP VHASRPALGR
 RRRGRRRGRA AHDGHWIRKL LAVARRRERH GQVLVAVARE LGVLEVLLQR
 FQALYARVCH LADLARVVHL PWLLVERLVQ PHASLRRCQV DEAVAHITLV
 VEIYGEVDEI VGGEGLLLAA RHDPLVGLDA VVVQHVEQQL LAVLVRYVPD
 HDGGPRIHLR SRRCLRVGAA AGLGLLEQEA RLPPALSSFP APAAAAPDAF
 LGLLPGRCRG RRGCRRRRHH DLAALGLALM ARSGIASLGR LVAARARLTG
 APPQKHHSFP RPYGTGRRRG CRRLRRRRLG RSPRRLKPRR SRRGGPVLVG
 CVPAPAGPGA GRARELDDEA AAEKLLAVEL GERARGVARV EEVHELVAGP
 LRRLPHILQV PEGLEDLAEV SDRDAGQGAQ DHQGAAVVAV VGAGGEVGPH
 GVPEDEAAAV VVGVGPVPGL GRRCRSPRRG YGGRRVELPE AALLAHVLLA
 DTGQGELGGA DVEEMDGGHA LGRHRARVDR ADRAEWRHRL RHVGLLRVRR
 DKAQHHDAAR RAIDHRRRRW QGVPRLRRHG HRHRRHWLHG YLRRRRHRHG
 WIWVGAVLLR KRLRIGGTDR RVELLSCGIE KVTRGFREPT HSTPSPT

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          797 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 148 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 155   Charge diffirence(C-N):  8.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   9
     Peak Value of UR:   3.18
     Net Charge of CR: 1
     Discriminant Score:      7.41
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 0.22
     Possible cleavage site: 38
>>> Seems to have a cleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 39
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -3.08  threshold: -2.0
     INTEGRAL    Likelihood = -3.08   Transmembrane  148 - 164 ( 147 - 164)
     PERIPHERAL  Likelihood = -0.69
     modified ALOM score:   0.72
>>> Seems to be a Type Ia membrane protein
     The cytoplasmic tail is from 165 to 797 (633 Residues)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
(13) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 13
     Uncleavable? Ipos set to: 23
Discrimination of mitochondrial target seq.: 
     negative (-2.09)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.36 at (63)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.91
     score from the 1-31 region:  5.34
Chloroplast protein?  Status: negative (-4.40)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 446(797), count: 2   ARL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:  -2.24
     AAC not from the N-term., score modified
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -8.22  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 126 (4) RRRP
     Found: pos: 179 (5) RRRR
     Found: pos: 180 (3) RRRH
     Found: pos: 199 (5) RRRR
     Found: pos: 414 (5) RRRR
     Found: pos: 415 (3) RRRH
     Found: pos: 474 (5) RRRR
     Found: pos: 486 (4) KPRR
     Found: pos: 714 (3) HRRR
     Found: pos: 715 (5) RRRR
     Found: pos: 731 (3) RHRR
     Found: pos: 742 (5) RRRR
     Found: pos: 743 (3) RRRH
     Found: pos: 744 (3) RRHR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 164 (3) RR VGGRSGRRRA VRGRR
     Found:  pos: 471 (3) RR LRRRRLGRSP RRLKP
     Found:  pos: 474 (3) RR RRLGRSPRRL KPRRS
     Found:  pos: 475 (3) RR RLGRSPRRLK PRRSR
     Found:  pos: 476 (4) RR LGRSPRRLKP RRSRR
Final Robbins Score (nucleus):  0.24
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.11)
Type Ia is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.460(Affirmative) < succ>
                          nucleus --- Certainty= 0.240(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.100(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----