Query Information

ORIGIN plant
BEGIN
>MYSEQ
 YALYRFKWPI YRFIIVISMC KHCTVALSLV VLEHLEVGST FAYGHGRGDD
 AKTLAELGFQ IGDYLSVAIM AGGPQVPATA PDITDVMKNA LVATSKGVSV
 SARKWNQNCL CRYYQSCLDT LNDQGVWYSF LLVWCLSAVP GVSNPLSCTS
 MAPCDGLNGI PRGGQDTHYL NHYPNLNYLN LYPTYPNLNP NTSLDPRSQN
 PKLYWVPGNM SRCLNYPKL

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          219 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 14 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 21   Charge diffirence(C-N): -3.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:   3.01
     Net Charge of CR: 2
     Discriminant Score:      5.41
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.98
     Possible cleavage site: 28
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -3.24  threshold: -2.0
     INTEGRAL    Likelihood = -3.24   Transmembrane   14 -  30 (  13 -  32)
     PERIPHERAL  Likelihood = -0.90
     modified ALOM score:   0.75
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 13 (13 Residues)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 14
     YRFIIV
Discrimination of mitochondrial target seq.: 
     positive ( 2.20)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.19 at (65)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.03
     score from the 1-31 region:  3.72
Chloroplast protein?  Status: negative (-8.68)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 5%
     Larger value (>30%) is favared for ER memb. proteins
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 14)  from: 23  to: 32  Score:  6.5
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(219), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -1.15
Peroxisomal proteins?   Status: negative
Checking the consensus for Golgi
Checking the consensus for Golgi
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -0.78  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.685(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.640(Affirmative) < succ>
                       Golgi body --- Certainty= 0.370(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----