Query Information

ORIGIN plant
BEGIN
>MYSEQ
 WPIHGHSALY IHDTEDARTA RNHNCARRLP RRRRMFPSGH PSSPHHQAVN
 GSFYAGELAG RPRRTGKRSS GRDLYAADRN QADQPGVRAQ DGHHQLQFEQ
 IGKRARPVPA RPRRIHMATC GYAGSPKRTG RAQIEGLSSG EADPAKATSF
 HARQERGHSS RVSSLGRGWI YRSITPRVVS QPCSCTQKES GLAHVRRLK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          199 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   5
     Peak Value of UR:   0.07
     Net Charge of CR: 2
     Discriminant Score:    -14.82
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.84
     Possible cleavage site: 60
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  13.42  threshold: -2.0
     PERIPHERAL  Likelihood = 13.42
     modified ALOM score:  -3.58
(12) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 12
     Uncleavable? Ipos set to: 22
Discrimination of mitochondrial target seq.: 
     negative (-4.62)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.17 at (47)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  4.86
Chloroplast protein?  Status: negative (-6.89)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(199), count: 0   
Amino Acid Composition Tendency for Peroxisome:   0.16
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.087
Amino acid composition tendency for vacuolar proteins
     Score: -8.79  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  29 (4) PRRR
     Found: pos:  30 (5) RRRR
     Found: pos:  60 (4) RPRR
     Found: pos: 110 (4) RPRR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.70)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.700(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.087(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----