Query Information

ORIGIN plant
BEGIN
>MYSEQ
 WNISGDPCTG IATDGTVIED NGNFNPGIKC ECSDQNNITV CHVTKLKIYA
 LNAVGPIPQE LQNLTRLINL DLSKNYLTGS LPSFLGNLTA MQYMTLGTNA
 LSGSVPKELG NLVNLVSLGF GSNYLNGPLP WELGNLAKLE QFHETLVADV
 GENKTFVDGD VGSVLVGGGV GGAFVGVPFS PYMCIATLLV ILLLFHLFLP
 LLVVVPVTVT CIWTFSNKVI GLTTKKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          229 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 161 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 168   Charge diffirence(C-N):  3.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:   1.21
     Net Charge of CR: -1
     Discriminant Score:     -8.26
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -5.44
     Possible cleavage site: 50
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value: -13.59  threshold: -2.0
     INTEGRAL    Likelihood =-13.59   Transmembrane  189 - 205 ( 173 - 217)
     PERIPHERAL  Likelihood = -1.65
     modified ALOM score:   2.82
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 206 to 229 (25 Residues)
( 5) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 5
     Uncleavable? Ipos set to: 15
Discrimination of mitochondrial target seq.: 
     negative (-5.19)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.55 at (67)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  4.89
     score from the 1-31 region:  3.46
Chloroplast protein?  Status: negative (-3.13)

*** Reasoning Step: 2

> Relative position of the end of the tail: 89%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.563
SKL motif (signal for peroxisomal protein): 
     pos: 137(229), count: 1   AKL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -0.59
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score:  1.05  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 224 (5) KKKK
     Found: pos: 225 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.563(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.300(Affirmative) < succ>
                          nucleus --- Certainty= 0.300(Affirmative) < succ>


----- The End -----