Query Information

ORIGIN plant
BEGIN
>MYSEQ
 VTHGRTQCSR FDLHGIPDQD FGYFDSSYKK GDKDVQSAME SPRKSNLGKR
 RRASHRFSPP FPIFLNLEDE IIFKGGRICN THFVICKYQR NNFSLCGFNL
 YASSYVNTIF KSKLIKEYMP SKLCIMLDIV FVECILLYKK IYKYIKREYI
 NTMRIELKGT LEIWRKGIKG IKYKQHICDY TSKIGTFYFT IIEDKLAQDL
 NLNLNLKWNL KTKRKRKTKK KK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          222 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 121 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 128   Charge diffirence(C-N):  4.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   1
     Peak Value of UR:  -0.14
     Net Charge of CR: 3
     Discriminant Score:    -16.86
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -10.15
     Possible cleavage site: 15
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -4.35  threshold: -2.0
     INTEGRAL    Likelihood = -4.35   Transmembrane  121 - 137 ( 119 - 141)
     PERIPHERAL  Likelihood =  7.32
     modified ALOM score:   0.97
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 138 to 222 (86 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 12
     SRFDLH
Discrimination of mitochondrial target seq.: 
     negative (-4.89)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.19 at (57)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.09
     score from the 1-31 region:  5.35
Chloroplast protein?  Status: negative (-4.71)

*** Reasoning Step: 2

> Relative position of the end of the tail: 62%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.194
SKL motif (signal for peroxisomal protein): 
     pos: 121(222), count: 2   SKL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   2.09
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.345
Amino acid composition tendency for vacuolar proteins
     Score:  1.75  Status: notclr
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  20.3% ( 0.31)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  48 (5) KRRR
     Found: pos: 212 (5) KRKR
     Found: pos: 213 (5) RKRK
     Found: pos: 218 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.50)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
                          nucleus --- Certainty= 0.657(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.541(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.200(Affirmative) < succ>


----- The End -----