Query Information

ORIGIN plant
BEGIN
>MYSEQ
 VTMARHTISI CSKSSTNCYV KKPTKLFPKP NEKLKFTIPI VSFFLQISRT
 RLFLRGDLRP KSDFGEKSKS VPNELYFGTH HLLKINRNIK TILVKVKKKF
 SLISRNNEWI IPFDCINIFL RVKVTHDRKK IHYFPLKSSN EYSLLNEILT
 KILKTIFSKT FHVCIAYLKI FKRGKIISKK NTCILYWIFY LFVVNSIKVN
 LEKLKLGFKT EMGKETKKKK 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          220 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 183 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 190   Charge diffirence(C-N): -4.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   6
     Peak Value of UR:   0.56
     Net Charge of CR: 2
     Discriminant Score:    -11.11
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.6
     Possible cleavage site: 50
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.70  threshold: -2.0
     PERIPHERAL  Likelihood = -1.70
     modified ALOM score:  -0.56
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 57
     LRGDLR
Discrimination of mitochondrial target seq.: 
     positive ( 2.59)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.91 at (29)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.99
     score from the 1-31 region:  7.17
Chloroplast protein?  Status: negative (-2.21)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 57)  from: 36  to: 45  Score:  5.0
Mitochondrial matrix?  Score:  0.48
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(220), count: 0   
Amino Acid Composition Tendency for Peroxisome:   2.75
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.434
Amino acid composition tendency for vacuolar proteins
     Score:  3.86  Status: positive
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  21.4% ( 0.37)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 216 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.72
Nuclear Signal   Status: notclr ( 0.42)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

       mitochondrial matrix space --- Certainty= 0.669(Affirmative) < succ>
                          nucleus --- Certainty= 0.634(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.434(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.359(Affirmative) < succ>


----- The End -----