Query Information

ORIGIN plant
BEGIN
>MYSEQ
 VRQAALAEEA ALGFFSGDFG NSPDCPVCTG LSGEPTVGRA NGRPRDRRAT
 RGRANGRRCA PDCPVRPTTE GKNSLPDVLS TAPRCLGAIK GTPWRMEEYN
 QAFLQPFAPR HSSAFDSLHI ELLWSCVLFE LCCELLLLLV CVLLSFEVLC
 ASLIPSPAPF SVNIFCKGER LQIVEIPRKR DEKKSKNTVV FKLIIGSLER
 SVQLSSVGTP QRGSRQVLYL AEPRDKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          229 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 135 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 142   Charge diffirence(C-N):  2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:   1.43
     Net Charge of CR: -1
     Discriminant Score:     -6.47
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.24
     Possible cleavage site: 42
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value: -11.36  threshold: -2.0
     INTEGRAL    Likelihood =-11.36   Transmembrane  135 - 151 ( 120 - 158)
     PERIPHERAL  Likelihood =  0.85
     modified ALOM score:   2.37
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 152 to 229 (79 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 4
     VRQAAL
Discrimination of mitochondrial target seq.: 
     negative (-2.24)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.19 at (54)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  7.93
Chloroplast protein?  Status: negative (-4.49)

*** Reasoning Step: 2

> Relative position of the end of the tail: 66%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.474
SKL motif (signal for peroxisomal protein): 
     pos: -1(229), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -3.59
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score: -2.16  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 176 (4) PRKR
     Found: pos: 225 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.474(Affirmative) < succ>
                          nucleus --- Certainty= 0.300(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.200(Affirmative) < succ>


----- The End -----