Query Information

ORIGIN plant
BEGIN
>MYSEQ
 VRFRRTYSPL RRPRRPGVAE RPNYSSLSAV TPQGPHTIGI TRQSLRSSLI
 KPLNQDQIPQ THSKVSHRRN TIKQENLNLK YWNYYINQSF SATDISSQNK
 LQQIIDIVES KDGKAFPTTP PAPLRSEQHP IRPSNPVAGW ANSHHQPYHE
 AYLQKIMPLA RLSILILSTY PVGIYSSTSR RRA

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          183 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   0
     Peak Value of UR:  -0.40
     Net Charge of CR: 4
     Discriminant Score:    -18.33
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.33
     Possible cleavage site: 39
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   0.85  threshold: -2.0
     PERIPHERAL  Likelihood =  0.85
     modified ALOM score:  -1.07
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 71
     RRNTIK
Discrimination of mitochondrial target seq.: 
     positive ( 2.29)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.83 at (70)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.84
     score from the 1-31 region:  7.98
Chloroplast protein?  Status: negative (-4.82)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 71)  from: 17  to: 19  Score:  2.5
Mitochondrial matrix?  Score:  0.45
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 160(183), count: 1   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -2.83
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -8.82  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  10 (4) RRPR
     Found: pos:  11 (4) RPRR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.72
Nuclear Signal   Status: notclr ( 0.42)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

       mitochondrial matrix space --- Certainty= 0.630(Affirmative) < succ>
                          nucleus --- Certainty= 0.420(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.329(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.329(Affirmative) < succ>


----- The End -----