Query Information

ORIGIN plant
BEGIN
>MYSEQ
 VPPKAEDVAD VRDGHDFFRK TTGRNPGPEH VVKDAHGAGV LRPCLRGTVE
 CRYHEREDKD TRPRNPGKYS TPLMCRSLAH PGPRRPAPLE RPSLILKVRL
 RFGTDSANAP QESRKKQVLS FISDQINRKK RPFSLGAALC SASTNESFRW
 NRTTRAGMRG AEGSYLIAQL CSGREGAIDP LLSFLKKKKK K

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          191 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   1
     Peak Value of UR:  -0.66
     Net Charge of CR: -2
     Discriminant Score:    -23.87
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.24
     Possible cleavage site: 51
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   7.85  threshold: -2.0
     PERIPHERAL  Likelihood =  7.85
     modified ALOM score:  -2.47
( 5) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 5
     Uncleavable? Ipos set to: 15
Discrimination of mitochondrial target seq.: 
     negative (-5.64)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.18 at (44)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  2.11
Chloroplast protein?  Status: negative (-10.03)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(191), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -2.70
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score: -1.92  Status: negative
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  20.4% ( 0.32)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 127 (5) RKKR
     Found: pos: 128 (4) KKRP
     Found: pos: 185 (5) KKKK
     Found: pos: 186 (5) KKKK
     Found: pos: 187 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 113 (3) RK KQVLSFISDQ INRKK
     Found:  pos: 114 (4) KK QVLSFISDQI NRKKR
Final Robbins Score (nucleus):  0.80
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.90)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.986(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----