Query Information

ORIGIN plant
BEGIN
>MYSEQ
 VLPVIYLLFE LLRWLVILHT QLEEERRERH EMEARMMAER EARLADHKRM
 AEMFQYMQSL GAAQGFTPPP PLFPAVDPAI FHTPVSIKIV VRCWCIWYNT
 CNLFSVQGQS GAASNNPHEG FSPTHQSNRP PPELCFQCLD FRTCVEYLCL
 CWILMSELET YETYVLDTYV CVENLNIYVC VGYLCLYLCI YVMSGA

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          196 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 2 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 9   Charge diffirence(C-N): -1.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:   2.31
     Net Charge of CR: -1
     Discriminant Score:     -2.10
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.96
     Possible cleavage site: 18
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -5.26  threshold: -2.0
     INTEGRAL    Likelihood = -5.26   Transmembrane  177 - 193 ( 174 - 195)
     INTEGRAL    Likelihood = -4.30   Transmembrane    2 -  18 (   1 -  20)
     PERIPHERAL  Likelihood =  2.17
     modified ALOM score:   1.15
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 15
     LRWLVI
Discrimination of mitochondrial target seq.: 
     notclr ( 1.40)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  5.76 at (25)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region: -0.35
Chloroplast protein?  Status: negative (-12.50)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 15)  from: 1  to: 9  Score:  5.5
Mitochondrial inner membrane?  Score:  0.46
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.230
SKL motif (signal for peroxisomal protein): 
     pos: 42(196), count: 1   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -0.66
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -3.14  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.590(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.300(Affirmative) < succ>


----- The End -----