Query Information

ORIGIN plant
BEGIN
>MYSEQ
 VGEGVEWVGS RKPRVTFSMP QLRSSTLRSV PPILYSRFRN SYTAPTHIQP
 CRCLRRRKPC YSQCLRWRWP WRRSRGTPCQ RRRRWSMARR AASWCWALSR
 RTRRRPTWRR RWRHSARSAR STRARWRPRA WPPSISSTSA PPSSPPVSAS
 STCASRAASG SSTRRPPPRR GLLHRRPRPG TGPTPTTTAA ASSSGTPCGP
 TSPPAPTTAT TAAPWWSAPC PASRSLTSAK SSRPSGTRMG SRRSGPATSS
 WTSSTRATPR ARSPSSTARS FSAAASSSSS RALPAPGPAG AGTHPTSTGP
 PRRLRRGFKR RGDRPNRRRL SRRHPRRRPV PGRGKEWCFG GAPVSLARAA
 TSRPREAMPE RAMSARPRAA RSWWRRRRHP RRPRQRPGSK PRKASGAAAA
 GAGKDERAGG RRASCSRSPR PAAAPTRKQR RLRRWIRGPP SSGTYRTSTA
 RSCCSTCWTT TASNPTSGSW RAARSSPSPP TISSTSPIST TSVMWATASS
 THRRRLACGC TRRSTSSHGR CTTRARSAKH TRAYKAWKRR STSRTPSSRA
 TATSTCPWRS RRRATARSLR IQCPSWAARP RRRPRRRLPR AGRLAWTGLG
 RSCRRRRHPR TARRRPLRPP TRRPNTTRRR RRETSGSQAS CGGLATTTSW
 LRSLQLGGRR LAQQRSAWRG VASRSAEAVV TSAKLFTDPR SLLHAQAKQV
 KWTGTLWRSK SELMFVGSTL RTAGVESCSF GFWFEVSYYL FLNLELLAAL
 GLLGGERACR GLSIRGSSCA RSLHNKIVIS TALQ

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          784 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 739 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 746   Charge diffirence(C-N):  4.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   0
     Peak Value of UR:   0.76
     Net Charge of CR: -1
     Discriminant Score:    -13.54
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -5.27
     Possible cleavage site: 53
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -0.22  threshold: -2.0
     PERIPHERAL  Likelihood = -0.22
     modified ALOM score:  -0.86
( 2) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 2
     Uncleavable? Ipos set to: 12
Discrimination of mitochondrial target seq.: 
     negative (-2.79)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.29 at (64)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  6.98
Chloroplast protein?  Status: negative (-5.41)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 683(784), count: 1   AKL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   1.58
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.278
Amino acid composition tendency for vacuolar proteins
     Score: -13.44  Status: negative
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  22.4% ( 0.42)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  10 (4) RKPR
     Found: pos:  54 (5) RRRK
     Found: pos:  55 (4) RRKP
     Found: pos:  80 (5) RRRR
     Found: pos: 102 (4) RRRP
     Found: pos: 174 (4) RRPR
     Found: pos: 324 (4) PRRR
     Found: pos: 325 (4) RRRP
     Found: pos: 374 (5) RRRR
     Found: pos: 375 (3) RRRH
     Found: pos: 380 (4) RRPR
     Found: pos: 389 (4) KPRK
     Found: pos: 501 (3) HRRR
     Found: pos: 578 (4) RPRR
     Found: pos: 579 (4) PRRR
     Found: pos: 580 (4) RRRP
     Found: pos: 581 (4) RRPR
     Found: pos: 582 (4) RPRR
     Found: pos: 583 (4) PRRR
     Found: pos: 603 (5) RRRR
     Found: pos: 604 (3) RRRH
     Found: pos: 612 (4) RRRP
     Found: pos: 627 (5) RRRR
     Found: pos: 628 (5) RRRR
Checking the 7 residue pattern for Nuclear Targeting
     Found:  pos: 300 (5) PRRLRRG
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:  88 (4) RR AASWCWALSR RTRRR
     Found:  pos: 301 (3) RR LRRGFKRRGD RPNRR
     Found:  pos: 304 (3) RR GFKRRGDRPN RRRLS
     Found:  pos: 309 (3) RR GDRPNRRRLS RRHPR
     Found:  pos: 376 (3) RR HPRRPRQRPG SKPRK
     Found:  pos: 613 (3) RR PLRPPTRRPN TTRRR
Final Robbins Score (nucleus):  0.80
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.90)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.988(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.494(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----