Query Information

ORIGIN plant
BEGIN
>MYSEQ
 TRGATEARDG RIMTSSACGH LPFLILISTI FVRIPGTRRR PRRYSENSLA
 GESRRQDTWT TPMVRSTSRN PLLARQGARR RYRARWRKLR RPCSIMRRWP
 SSLSASRRRL KVARDSQARP RQRRSARASN VVRLVISLLT VPKMKVTRKR
 GTKGRRRSIT RRPRARHIER SGIWIAPPPT PTMKDSPPPP STSHLSSPTS
 VTHALWQGRR RV

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          212 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 16 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 23   Charge diffirence(C-N):  4.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:   2.60
     Net Charge of CR: 1
     Discriminant Score:      3.08
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.7
     Possible cleavage site: 29
... positive value of mtop ...
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.44  threshold: -2.0
     INTEGRAL    Likelihood = -2.44   Transmembrane   16 -  32 (  16 -  34)
     PERIPHERAL  Likelihood =  2.23
     modified ALOM score:   0.59
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 33 to 212 (181 Residues)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
 count: 1)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 45
     (count:1)
     PRRYSE
Discrimination of mitochondrial target seq.: 
     notclr (-0.33)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.99 at (26)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.09
     score from the 1-31 region:  6.81
Chloroplast protein?  Status: negative (-5.02)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

> Relative position of the end of the tail: 15%
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 45)  from: 21  to: 32  Score:  7.0
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 193(212), count: 1   SHL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:  -1.58
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -14.45  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  37 (4) RRRP
     Found: pos:  38 (4) RRPR
     Found: pos:  39 (4) RPRR
     Found: pos: 160 (4) RRPR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 106 (3) RR RLKVARDSQA RPRQR
     Found:  pos: 107 (3) RR LKVARDSQAR PRQRR
     Found:  pos: 147 (3) RK RGTKGRRRSI TRRPR
     Found:  pos: 148 (3) KR GTKGRRRSIT RRPRA
Final Robbins Score (nucleus):  0.24
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.11)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.820(Affirmative) < succ>
                          nucleus --- Certainty= 0.240(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.190(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----