Query Information

ORIGIN plant
BEGIN
>MYSEQ
 TNHIKEDKHQ LKSSKDQVES PAESRSTVLD EVFPYYTLPC HNLYLPPYLI
 HNKWLDIDIH IKNFKQNFGF QNQMRNNSRP NLLYPFSYKS RGRDFCGGNL
 HLKSFWGQKK SNSISQKFEN NISNELVIIM EILNKHLNCL YINDVLLEII
 CVRYSYLKTF ASINKVNNTN KYIYTYLHLC SFRWCILPCI LPVNVTTSNK
 KKKKL

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          205 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 179 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 186   Charge diffirence(C-N):  2.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:  -0.96
     Net Charge of CR: 2
     Discriminant Score:    -22.99
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -7.71
     Possible cleavage site: 34
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   0.37  threshold: -2.0
     PERIPHERAL  Likelihood =  0.37
     modified ALOM score:  -0.97
( 5) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 5
     Uncleavable? Ipos set to: 15
Discrimination of mitochondrial target seq.: 
     negative (-6.35)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 14.23 at (54)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  9.59
Chloroplast protein?  Status: notclr (-0.19)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
     found at (68-70):  2.5
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-75  Score: 542.72
Chloroplast stroma?  Score: 0.4
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(205), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -2.23
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score:  2.11  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 199 (5) KKKK
     Found: pos: 200 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.60)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.600(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.400(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----