Query Information

ORIGIN plant
BEGIN
>MYSEQ
 THLLLPCHKA CVTLVGEERL VEGGGGESFI VGVGGGAIQI PLLSYMCLAL
 GLLVMLLLLP FVPLFLVTFI FGTVSNEMTN LTTFEARALL LCLGLAWLSL
 ATFKRRLEAL NDEGHLLIIE HGRLNLRHLA RRLLAPHCLA SKGLRLVDRT
 IQGVVHVSCL LDHHSPANEF SEYLLGRHLR VPGILTNIVH EMRIKNGKPH
 ADDVMIRPSR ASVAPYLV

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          218 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 46 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 53   Charge diffirence(C-N):  2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:   1.49
     Net Charge of CR: 3
     Discriminant Score:     -3.67
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 2.24
     Possible cleavage site: 60
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value: -11.04  threshold: -2.0
     INTEGRAL    Likelihood =-11.04   Transmembrane   46 -  62 (  36 -  75)
     PERIPHERAL  Likelihood = -1.97
     modified ALOM score:   2.31
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 63 to 218 (157 Residues)
(16) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 16
     Uncleavable? Ipos set to: 26
Discrimination of mitochondrial target seq.: 
     negative (-1.99)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.39 at (30)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.93
     score from the 1-31 region:  2.27
Chloroplast protein?  Status: negative (-6.33)

*** Reasoning Step: 2

> Relative position of the end of the tail: 28%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.462
SKL motif (signal for peroxisomal protein): 
     pos: -1(218), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -0.89
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score:  2.16  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.600(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.462(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.100(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----