Query Information

ORIGIN plant
BEGIN
>MYSEQ
 TAIGGGVSVA KAQRLWKAVV GPTSSPPPIE LFHTLSSKDR AITQRVHISW
 SEQGLSVRDL LFFAVFLSPI VQKKPKNSRL VLPQSYKPLS IYYYLVRACV
 FGCIGCVELL VLVYSFRVLS STTFWSPRNP PSPKTSLVRF CHHGYISYPI
 WKFKYNLESL SYLLSNGSDL ISKFVLSAPQ SSRFLDFLYQ DLLSLILRGC
 QYLYCLGCHS EKKGFRPLQK ETEEEEKKKN EKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          235 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 55 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 62   Charge diffirence(C-N):  4.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:   0.90
     Net Charge of CR: 1
     Discriminant Score:    -10.63
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.43
     Possible cleavage site: 43
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -6.53  threshold: -2.0
     INTEGRAL    Likelihood = -6.53   Transmembrane   98 - 114 (  94 - 120)
     INTEGRAL    Likelihood = -2.34   Transmembrane   55 -  71 (  55 -  71)
     PERIPHERAL  Likelihood =  2.28
     modified ALOM score:   1.41
>>> Likely a Type IIIb membrane protein (Nexo Ccyt)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 16
     QRLWKA
Discrimination of mitochondrial target seq.: 
     notclr ( 0.15)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.36 at (47)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  7.26
Chloroplast protein?  Status: negative (-3.32)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 16)  from: 60  to: 71  Score:  8.5
Mitochondrial inner membrane?  Score:  0.56
Mitochondrial intermemb.space?  Score:  0.09
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.281
SKL motif (signal for peroxisomal protein): 
     pos: 78(235), count: 1   SRL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   2.79
Peroxisomal proteins?   Status: positive
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -1.01  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  72 (4) KKPK
     Found: pos: 231 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.06)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

     mitochondrial inner membrane --- Certainty= 0.613(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.425(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>


----- The End -----