Query Information

ORIGIN plant
BEGIN
>MYSEQ
 STCPGELEWP SPETGPSIFC ISSQHIKEKL HRIREGVICC FDGIQKASAL
 FSSLQHNRSF FTTSEGYYEE PRSYWKDWKM GCRAQILYIC SIFDSVPGVS
 RLHCLDARGS GGGSKRRRSM DSVLWILGNL RSRSGCCVGF TFQGQNLLCG
 KTFLHKHCRV RSPASRSSEA KSNGNQKGYT QNFPGCFGSY QELQGGSEAK
 ISGGGSKSSF LRRI

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          214 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:   1.35
     Net Charge of CR: -2
     Discriminant Score:     -8.88
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.54
     Possible cleavage site: 49
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   3.07  threshold: -2.0
     PERIPHERAL  Likelihood =  3.07
     modified ALOM score:  -1.51
( 5) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 5
     Uncleavable? Ipos set to: 15
Discrimination of mitochondrial target seq.: 
     negative (-5.83)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  6.82 at (26)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.93
     score from the 1-31 region:  6.98
Chloroplast protein?  Status: negative (-2.90)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 100(214), count: 1   SRL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   3.14
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.486
Amino acid composition tendency for vacuolar proteins
     Score: -2.41  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 114 (5) KRRR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
                          nucleus --- Certainty= 0.300(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----