Query Information

ORIGIN plant
BEGIN
>MYSEQ
 SIFYGLSFHD LITACVSHFR SMYICLCCYQ RSCFCVTCSI KDLEGTDTTG
 KCFHSFIVLA SRSTQIVFGD LIVHFQEKYF YSNSLHFHIV NKFLGTQCSR
 ASKKHERFFL QVTSMPELFT RTFLLFYVLH NCQSFRNLTM LKSLRKQSFI
 YFMHPLCNLL EIIPIEKHFL FMVSSCIIIC EHHAKQLIKI YTTKLCIMLD
 FILCAFCDKK ILKTKKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          219 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 162 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 169   Charge diffirence(C-N):  3.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:   1.95
     Net Charge of CR: 0
     Discriminant Score:     -2.44
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 1.21
     Possible cleavage site: 14
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.17  threshold: -2.0
     PERIPHERAL  Likelihood = -1.17
     modified ALOM score:  -0.67
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 33
     QRSCFC
Discrimination of mitochondrial target seq.: 
     notclr ( 0.19)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.24 at (35)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  7.14
Chloroplast protein?  Status: negative (-4.70)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 33)  from: 56  to: 60  Score:  5.0
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(219), count: 0   
Amino Acid Composition Tendency for Peroxisome:   5.39
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score:  1.74  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 214 (5) KKKK
     Found: pos: 215 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.60)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
                          nucleus --- Certainty= 0.600(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.436(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.119(Affirmative) < succ>


----- The End -----