Query Information

ORIGIN plant
BEGIN
>MYSEQ
 SHRRWCIGCG AASLEGCSRA VNVVSSPNRV IPHPLIERSG NHPTGAHQLV
 IRARFIGERF TFLRCFLISY SPEKAKKISF TAILTIEHLL VLLSGLLSFW
 LHRLCRVAGL SLFLSFEFYH VLVTTKSTIT KNISGSFLPP RIHIISDLEV
 IQSGKLILSS FQRIPYLKIR SERSAIIVEI SGLSILTRFV KSDFKGLLAI
 SLLFGLSKKR VAPAKRNRRR RKKKEKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          229 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 83 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 90   Charge diffirence(C-N):  0.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   10
     Peak Value of UR:   1.60
     Net Charge of CR: 3
     Discriminant Score:     -1.96
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.63
     Possible cleavage site: 20
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -3.35  threshold: -2.0
     INTEGRAL    Likelihood = -3.35   Transmembrane   83 -  99 (  80 - 101)
     PERIPHERAL  Likelihood = -0.59
     modified ALOM score:   0.77
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 82 (82 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 143
     PRIHII
Discrimination of mitochondrial target seq.: 
     notclr ( 0.86)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.22 at (50)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  4.95
     score from the 1-31 region:  9.93
Chloroplast protein?  Status: positive ( 2.18)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 35%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 143)  from: 6  to: 14  Score:  5.5
Mitochondrial inner membrane?  Score:  0.31
Checking apolar signal for intrachloroplastic sorting
     found at (89-99):  7.5
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-14  Score: 9666.56
Chloroplast thylakoid memb.?  Score: 0.423
SKL motif (signal for peroxisomal protein): 
     pos: -1(229), count: 0   
Amino Acid Composition Tendency for Peroxisome:   2.07
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.343
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score:  0.72  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 217 (5) RRRR
     Found: pos: 218 (5) RRRK
     Found: pos: 219 (5) RRKK
     Found: pos: 220 (5) RKKK
     Found: pos: 225 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 207 (5) KK RVAPAKRNRR RRKKK
     Found:  pos: 208 (4) KR VAPAKRNRRR RKKKE
Final Robbins Score (nucleus):  0.80
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.55)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                          nucleus --- Certainty= 0.910(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.650(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.607(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.436(Affirmative) < succ>


----- The End -----