Query Information

ORIGIN plant
BEGIN
>MYSEQ
 SFFFFFFQLQ VYVTFTGKIQ GKIHQRKDQH KCKYVYIYLF VLFTLLIDYA
 NVFKYEYRTH IIYSKRTSFM YRQFKCLFKI SIIITNSFEM LFSNFYWEIL
 FDFFCPQNDF RCYKFYPPQK SRPRDLEKGY NKFGLEFFLI WFWKPKFCLK
 FFIWISMSSH LLCQMRYGYG KYRLWQGRVG KTSSRTVDLD SAGDSTSFED
 LSCLSSLMWL V

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          211 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 37 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 44   Charge diffirence(C-N): -4.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   16
     Peak Value of UR:   2.14
     Net Charge of CR: 0
     Discriminant Score:      1.62
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -1.86
     Possible cleavage site: 21
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.97  threshold: -2.0
     INTEGRAL    Likelihood = -2.97   Transmembrane   37 -  53 (  34 -  53)
     PERIPHERAL  Likelihood =  0.85
     modified ALOM score:   0.69
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 36 (36 Residues)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 28
     QRKDQH
Discrimination of mitochondrial target seq.: 
     notclr ( 0.30)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.91 at (70)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  3.83
Chloroplast protein?  Status: negative (-8.67)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 17%
     Larger value (>30%) is favared for ER memb. proteins
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 28)  from: 35  to: 47  Score:  8.5
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 159(211), count: 1   SHL
     SKL score (peroxisome):  0.1
Amino Acid Composition Tendency for Peroxisome:  17.68
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.320
Checking the consensus for Golgi
Checking the consensus for Golgi
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -2.74  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.685(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.640(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.388(Affirmative) < succ>
                       Golgi body --- Certainty= 0.370(Affirmative) < succ>


----- The End -----