Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RVFFFFFNFN FKFMLHFKLR VKYRVKYTNE KININANMCI YICLYYLLYL
 ITQMFSNMNT AHILFIPREH HFKCIDNLNV YSKFPLLLIH FKRCYFQISI
 GRYFSLISFA NPKIRILGVI NSTPLNRNLV LEICKKRDII SLVNFSFSFG
 FGNQSFVSFL YGYLCLAIYY VRGTVMASIG CGKEEYNKER LHLELWILIP
 LAIQLDPLKT VDVYPLCGW

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          219 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 39 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 46   Charge diffirence(C-N): -3.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   10
     Peak Value of UR:   2.19
     Net Charge of CR: 1
     Discriminant Score:      0.81
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -4.12
     Possible cleavage site: 56
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.12  threshold: -2.0
     PERIPHERAL  Likelihood = -1.12
     modified ALOM score:  -0.68
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 26
     YRVKYT
Discrimination of mitochondrial target seq.: 
     positive ( 2.72)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.89 at (27)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  1.51
Chloroplast protein?  Status: negative (-10.15)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 26)  from: 38  to: 51  Score:  6.5
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(219), count: 0   
Amino Acid Composition Tendency for Peroxisome:   6.39
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.320
Amino acid composition tendency for vacuolar proteins
     Score:  4.18  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.550(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.320(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>
                          outside --- Certainty= 0.100(Affirmative) < succ>


----- The End -----