Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RTLHPRHASL SQLRMARLLR KLKKSRQLQA PMVVSKPRKP EQMTPLPLSS
 QSRRPPCSRR MAGQHDPASL HQYALGPRTW LQLQWWIRMS QVQRCPCDKI
 WRTTRCMKND WFRLCLFLVC VGLQSSHEYL MLTTVPSRNL NLFGHYLKKK
 KK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          152 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   6
     Peak Value of UR:   0.32
     Net Charge of CR: 4
     Discriminant Score:    -11.43
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -5.38
     Possible cleavage site: 52
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   2.76  threshold: -2.0
     PERIPHERAL  Likelihood =  2.76
     modified ALOM score:  -1.45
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 96
     QRCPCD
Discrimination of mitochondrial target seq.: 
     positive ( 3.70)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  6.71 at (43)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region:  8.23
Chloroplast protein?  Status: negative (-5.53)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 96)  from: 30  to: 34  Score:  2.5
Mitochondrial matrix?  Score:  0.61
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: 16(152), count: 1   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -0.25
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.034
Amino acid composition tendency for vacuolar proteins
     Score: -13.13  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  35 (4) KPRK
     Found: pos: 147 (5) KKKK
     Found: pos: 148 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:  22 (3) KK SRQLQAPMVV SKPRK
Final Robbins Score (nucleus):  0.36
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.77)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.853(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.792(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.470(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.470(Affirmative) < succ>


----- The End -----