Query Information
ORIGIN plant
BEGIN
>MYSEQ
RRRRRRRLHQ VDNQSTPPSS TMRKWRSSSR VSAKSSNKGG GKTTSPAPRR
FATSVVSPVI LLLNVLYLVT VTEVTTRRGD ERKRRGITRR RAAMPMFVAN
GTPTRAQATP PTTRTPPTSP SPRDSSSPTS ATSASWQRTA KRRLNLNPPL
NMNPLVMTML VMRKIICVPF LPTLTLKKKN MNWLVLFMKT TTFWIPKRIV
Result Information
PSORT --- Prediction of Protein Localization Sites
version 6.4(WWW)
MYSEQ 200 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5
*** Reasoning Step: 1
Preliminary Calculation of ALOM (threshold: 0.5)
count: 2
Position of the most N-terminal TMS: 55 at i=1
MTOP: membrane topology (Hartmann et al.)
I(middle): 62 Charge diffirence(C-N): -1.0
McG: Examining signal sequence (McGeoch)
Length of UR: 2
Peak Value of UR: -0.64
Net Charge of CR: 8
Discriminant Score: -16.71
GvH: Examining signal sequence (von Heijne)
Signal Score (-3.5): -7.95
Possible cleavage site: 61
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
count: 2 value: -7.38 threshold: -2.0
INTEGRAL Likelihood = -7.38 Transmembrane 55 - 71 ( 50 - 76)
INTEGRAL Likelihood = -3.40 Transmembrane 155 - 171 ( 154 - 172)
PERIPHERAL Likelihood = 5.99
modified ALOM score: 1.58
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Gavel: Examining the boundary of mitochondrial targeting seq.
motif at: 52
RRFATS
Discrimination of mitochondrial target seq.:
positive ( 2.15)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins
Hmax: 6.82 at (61)
Disc.Score from Amino Acid Composition (chloroplast)
score from the 3-11 region: -4.62
score from the 1-31 region: 9.43
Chloroplast protein? Status: negative (-8.00)
*** Reasoning Step: 2
Type IIIa or IIIb is favored for ER memb. proteins
KDEL Count: 0
Checking apolar signal for intramitochondrial sorting
(Gavel position 52) from: 51 to: 71 Score: 7.5
Mitochondrial inner membrane? Score: 0.80
Mitochondrial intermemb.space? Score: 0.09
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.? Score: 0.315
SKL motif (signal for peroxisomal protein):
pos: -1(200), count: 0
Amino Acid Composition Tendency for Peroxisome: -1.97
Peroxisomal proteins? Status: negative
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
Score: -11.77 Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Found: pos: 1 (5) RRRR
Found: pos: 2 (5) RRRR
Found: pos: 3 (5) RRRR
Found: pos: 81 (5) RKRR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Found: pos: 76 (3) RR GDERKRRGIT RRRAA
Found: pos: 162 (3) RK IICVPFLPTL TLKKK
Final Robbins Score (nucleus): 0.48
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal Status: negative ( 0.08)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..
----- Final Results -----
mitochondrial inner membrane --- Certainty= 0.863(Affirmative) < succ>
plasma membrane --- Certainty= 0.600(Affirmative) < succ>
nucleus --- Certainty= 0.480(Affirmative) < succ>
Golgi body --- Certainty= 0.400(Affirmative) < succ>
----- The End -----