Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RRQHGMVRGQ GQDSLPSGGK WIQCAIYTER LDRTDRDQIA RPYPWRDRQA
 EFKASGEGFL LPSRCARKRW SRSSARARTR AHPPGKPEDG TGEGENRCRG
 REYQDSMQRV RGFVQRRGTQ VQRSLGSAAA EGRQVMHKLW VSFRCFLLFM
 LGCSFIYVSQ WAQIDVLWLL LCSRSV

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          176 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 140 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 147   Charge diffirence(C-N): -2.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:   0.57
     Net Charge of CR: 4
     Discriminant Score:    -10.29
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -10.31
     Possible cleavage site: 19
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.07  threshold: -2.0
     INTEGRAL    Likelihood = -2.07   Transmembrane  140 - 156 ( 139 - 159)
     PERIPHERAL  Likelihood =  5.14
     modified ALOM score:   0.51
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 139 (139 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 10
     VRGQGQ
Discrimination of mitochondrial target seq.: 
     negative (-5.82)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  6.16 at (42)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region:  4.81
Chloroplast protein?  Status: negative (-9.11)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 78%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.103
SKL motif (signal for peroxisomal protein): 
     pos: -1(176), count: 0   
Amino Acid Composition Tendency for Peroxisome:   4.60
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -15.30  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.850(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.440(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.103(Affirmative) < succ>


----- The End -----