Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RQSSWESKRS SFSIALTNSF NFSFAMLRLA KRERKLSSSS LALSSLEDSY
 LVEDLDLTFF LPSFAMRHLW PTLGKRSPLV TAMLAASSSS EESLELSSES
 HSRQTWASPP FFLYLFFSFR LPFLSSPRSL SLDIGHLAIK PGLPHLQTFL
 ERDLSFPLLC LRIWRKLLMT SAISSLSSLE ASIGCRLGVA SSFFSSV

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          197 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   18
     Peak Value of UR:   1.45
     Net Charge of CR: 2
     Discriminant Score:     -1.24
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.52
     Possible cleavage site: 25
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   3.29  threshold: -2.0
     PERIPHERAL  Likelihood =  3.29
     modified ALOM score:  -1.56
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 36
     ERKLSS
Discrimination of mitochondrial target seq.: 
     positive ( 3.21)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.48 at (53)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region: 14.80
Chloroplast protein?  Status: positive ( 2.10)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 36)  from: 57  to: 66  Score:  4.5
Mitochondrial matrix?  Score:  0.55
Checking apolar signal for intrachloroplastic sorting
     found at (79-86):  6.0
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-84  Score: 29491.20
Chloroplast stroma?  Score: 0.75
SKL motif (signal for peroxisomal protein): 
     pos: -1(197), count: 0   
Amino Acid Composition Tendency for Peroxisome:   6.65
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score:  2.86  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

               chloroplast stroma --- Certainty= 0.827(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.741(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.421(Affirmative) < succ>


----- The End -----