Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RPSLSYIPSI VTITLACNIT YPTRRREEGT SDGGEDDGGA GGSPGRSEDG
 GDVPVHAEPW CCTGLHSATS IVPCSPCYLP YSCEYQNCSM LVMHLVHMQS
 LLCAGPIWGG IQQPSRVQPN VAPVEPPTSM RVMFSVFRLQ NLCILMFVLD
 TYVRADLDLC SYLCLRELEY LCLCWIFMFV MISVMYICDV WC

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          192 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 132 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 139   Charge diffirence(C-N): -3.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   22
     Peak Value of UR:   2.49
     Net Charge of CR: 1
     Discriminant Score:      6.56
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.93
     Possible cleavage site: 16
>>> Seems to have an uncleavable N-term signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -8.44  threshold: -2.0
     INTEGRAL    Likelihood = -8.44   Transmembrane  171 - 187 ( 167 - 191)
     INTEGRAL    Likelihood = -2.13   Transmembrane  132 - 148 ( 132 - 149)
     PERIPHERAL  Likelihood =  1.22
     modified ALOM score:   1.79
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Rule: vesicular pathway 
Rule: vesicular pathway 
Rule: vesicular pathway 
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 27
     RRREEG
Discrimination of mitochondrial target seq.: 
     positive ( 1.82)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.18 at (51)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.90
     score from the 1-31 region:  5.71
Chloroplast protein?  Status: negative (-4.25)
Rule: vesicular pathway 
Rule: vesicular pathway 

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
Memb.protein with uncleavable signl is often at ER
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 27)  from: 10  to: 17  Score:  4.5
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(192), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -2.65
Peroxisomal proteins?   Status: negative
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -1.85  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.685(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.640(Affirmative) < succ>
                       Golgi body --- Certainty= 0.460(Affirmative) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.100(Affirmative) < succ>


----- The End -----