Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RPFLGKKVGV SLRVGFPTKR RVRKIKNATW FIEVEVPRIG CPTLLPFMFK
 KKIIKTLEKP TKRRSLGLSF SCGTLSVLVS GPSFTRSLLS ASSCLISCAY
 SFFTYLIQRL DQAVSNDRWH ATYLVSYPDA LLACICEGYV NLGQRYLFSM
 SEDSGITPRM EHYECMIELF SKHGFRIIAK PGLYSPTSSY SPVKVLVTLL
 LLLEVQFHFG WEFSV

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          215 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 193 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 200   Charge diffirence(C-N): -3.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   5
     Peak Value of UR:   1.29
     Net Charge of CR: 3
     Discriminant Score:     -5.67
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -8.69
     Possible cleavage site: 41
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.86  threshold: -2.0
     PERIPHERAL  Likelihood = -1.86
     modified ALOM score:  -0.53
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 25
     VRKIKN
Discrimination of mitochondrial target seq.: 
     negative (-1.01)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 12.18 at (31)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  1.95
Chloroplast protein?  Status: negative (-9.35)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(215), count: 0   
Amino Acid Composition Tendency for Peroxisome:   4.74
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -0.33  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:   5 (3) KK VGVSLRVGFP TKRRV
     Found:  pos:  49 (3) KK KIIKTLEKPT KRRSL
Final Robbins Score (nucleus):  0.80
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.70
Nuclear Signal   Status: notclr ( 0.40)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.880(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----