Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RNSFLRTLKI IRKLPKIFVN EVLIFRLIIT IFSLLFNYYL FLKIDIKHLV
 RNQMHAKKTM KINNTLKIYY PNYNIIYYFN WLLWCYKPTP LKKNLVLEIK
 KLPRIAEGIL LVHLLFPKWL HLPNDDSTEL CTCLYSVFCE MFPEFLGTPN
 KSNLHHSVLP WVTVLRVLLS TSVVTREIRH AHLIIYRVAL TGRLLRLFFP
 KSMGQYSEVI IFLPTFSFLS SVKLGRQSLL LQQQFS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          236 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 22 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 29   Charge diffirence(C-N):  1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:   2.12
     Net Charge of CR: 3
     Discriminant Score:     -0.73
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.58
     Possible cleavage site: 35
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -4.30  threshold: -2.0
     INTEGRAL    Likelihood = -4.30   Transmembrane   22 -  38 (  16 -  44)
     PERIPHERAL  Likelihood = -1.86
     modified ALOM score:   0.96
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 39 to 236 (199 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 53
     VRNQMH
Discrimination of mitochondrial target seq.: 
     positive ( 2.67)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.24 at (36)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.04
     score from the 1-31 region:  2.47
Chloroplast protein?  Status: negative (-8.05)

*** Reasoning Step: 2

> Relative position of the end of the tail: 16%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 53)  from: 27  to: 36  Score:  7.0
Mitochondrial inner membrane?  Score:  0.58
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.192
SKL motif (signal for peroxisomal protein): 
     pos: 181(236), count: 1   AHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   4.53
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -0.39  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.748(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.732(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.600(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.367(Affirmative) < succ>


----- The End -----