Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RKNQPEWTDC NRLLQRRVRE VRHHGIGRGA AFPACRLAEA VWGTHDSLRF
 PAIWLSTGEP FRLASGAPCG EDRYKNWPTE QMRRVPSQIN SSRNLCRNSA
 PYHLDIRAHV YDGNRYSLQV SSPGVLISLR SRTLVLLVCT VDRYYSVSPF
 ESAFRIIIII FLLSHLLFTM CSNFHQTNAC VGLCCDQTHC LQVFSVNNLE
 FQVGTVHQIK KMKSCCAKKK KK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          222 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 125 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 132   Charge diffirence(C-N): -2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:  -0.81
     Net Charge of CR: 0
     Discriminant Score:    -23.27
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.75
     Possible cleavage site: 43
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -8.12  threshold: -2.0
     INTEGRAL    Likelihood = -8.12   Transmembrane  156 - 172 ( 150 - 174)
     INTEGRAL    Likelihood = -2.07   Transmembrane  125 - 141 ( 125 - 141)
     PERIPHERAL  Likelihood =  5.04
     modified ALOM score:   1.72
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 3
     
Discrimination of mitochondrial target seq.: 
     notclr (-0.11)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.19 at (40)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  3.94
     score from the 1-31 region:  1.93
Chloroplast protein?  Status: negative (-6.83)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 3)  from: 29  to: 35  Score:  4.0
Mitochondrial inner membrane?  Score:  0.69
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.345
SKL motif (signal for peroxisomal protein): 
     pos: 164(222), count: 1   SHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -0.67
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -6.07  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 217 (5) KKKK
     Found: pos: 218 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.06)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

     mitochondrial inner membrane --- Certainty= 0.690(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.345(Affirmative) < succ>


----- The End -----