Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RHEVQFSIVR CPRRGTMRLH TLRGMKAEDI CIAFICIHSD ICRCGFILSE
 IRSTYTLHSY AYIEVIFAGI VDAGRCYRTY CGCSRKDDIG YIGTTKFYIY
 KYFSLFFNVD FNLWFKYLIR TISLLRCFIS LLHYSMQVLV AHQGEWDVAA
 HPPSLSCCPD TVMILETSCQ RMFVFLVVHT DFSSCCYGCL PIASRLLMSF
 MFSYHDIYLY FVASRFMQFS KEMMSKFKKK KK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          232 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 32 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 39   Charge diffirence(C-N):  1.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:  -0.00
     Net Charge of CR: 2
     Discriminant Score:    -16.26
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.31
     Possible cleavage site: 44
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.01  threshold: -2.0
     PERIPHERAL  Likelihood = -1.01
     modified ALOM score:  -0.70
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 25
     LRGMKA
Discrimination of mitochondrial target seq.: 
     notclr ( 1.28)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.57 at (41)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.03
     score from the 1-31 region:  2.74
Chloroplast protein?  Status: negative (-9.83)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 25)  from: 30  to: 37  Score:  7.0
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 194(232), count: 1   SRL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   6.31
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score:  0.97  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 227 (5) KKKK
     Found: pos: 228 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.60)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.748(Affirmative) < succ>
                          nucleus --- Certainty= 0.600(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.506(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.228(Affirmative) < succ>


----- The End -----