Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RHELLCSSPH SLNPSRHPPP ISSSPSDVLP RRIFFVWVQV PIGELVGGRK
 EERRNPRWWT SGSYLGICPN FRYLIYVGNE GSSSTSTFTL ILRLPKRIHD
 LIRNLMSKDV CFFYSCALVN SVHVVCLTFL CPFNNTYVFC HDTCLYTVAP
 SFMLFCKGDV VEILYMDVRS VV

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          172 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 115 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 122   Charge diffirence(C-N):  1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   5
     Peak Value of UR:   0.05
     Net Charge of CR: 2
     Discriminant Score:    -14.99
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.63
     Possible cleavage site: 14
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.34  threshold: -2.0
     INTEGRAL    Likelihood = -2.34   Transmembrane  115 - 131 ( 114 - 133)
     PERIPHERAL  Likelihood =  2.17
     modified ALOM score:   0.57
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 132 to 172 (42 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 34
     RRIFFV
Discrimination of mitochondrial target seq.: 
     negative (-5.36)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 12.00 at (31)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  2.93
     score from the 1-31 region: 12.24
Chloroplast protein?  Status: positive ( 3.81)

*** Reasoning Step: 2

> Relative position of the end of the tail: 76%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
     found at (88-92):  3.5
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-50  Score: 26.88
Chloroplast thylakoid memb.?  Score: 0.312
SKL motif (signal for peroxisomal protein): 
     pos: -1(172), count: 0   
Amino Acid Composition Tendency for Peroxisome:   2.03
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.337
Amino acid composition tendency for vacuolar proteins
     Score: -0.39  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.643(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.481(Affirmative) < succ>
      chloroplast thylakoid space --- Certainty= 0.481(Affirmative) < succ>


----- The End -----