Query Information

ORIGIN plant
BEGIN
>MYSEQ
 RELLLKETLS TLKFHREEKG LRLKENYHLA ILAHSRFWKE KAKPTSSYPI
 DYQMCMTYFT CHNSRSAEYP KNSYPWKNML MKILIRSTQS EFWKHLAELH
 GVKSLTCAKF SGVIIRKMKP LGKEKMNSEQ NSLSYSRKFL NLEDEILFKR
 GRFVTPKPSI KIINLDIILI RVINLFCLII YFHRFLCMHL ISYEMFNINS
 FKEILVIKLR KYSNYSKNKY FIYKYFWFSY NFSSKIS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          237 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 165 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 172   Charge diffirence(C-N): -1.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   5
     Peak Value of UR:   0.14
     Net Charge of CR: 0
     Discriminant Score:    -15.72
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.84
     Possible cleavage site: 35
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -6.37  threshold: -2.0
     INTEGRAL    Likelihood = -6.37   Transmembrane  165 - 181 ( 159 - 186)
     PERIPHERAL  Likelihood =  4.72
     modified ALOM score:   1.37
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 164 (164 Residues)
( 1) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 1
     Uncleavable? Ipos set to: 11
Discrimination of mitochondrial target seq.: 
     negative (-2.41)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.82 at (43)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.01
     score from the 1-31 region:  2.77
Chloroplast protein?  Status: negative (-8.18)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 69%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.275
SKL motif (signal for peroxisomal protein): 
     pos: -1(237), count: 0   
Amino Acid Composition Tendency for Peroxisome:   4.02
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.603
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score:  4.91  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 136 (3) RK FLNLEDEILF KRGRF
Final Robbins Score (nucleus):  0.60
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.20)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

 endoplasmic reticulum (membrane) --- Certainty= 0.850(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.603(Affirmative) < succ>
                          nucleus --- Certainty= 0.600(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.440(Affirmative) < succ>


----- The End -----