Query Information

ORIGIN plant
BEGIN
>MYSEQ
 QQDKELRKHA MDGSRRPPNA KVIYRSSSSR EQYSESQVHE DDARGGTRKI
 RRANDDQGGK IRGRRLEWSD QRAATPCSQG NEEQGGATEQ GGASGGRAQG
 DGPHHALQDG ARSQGTAKQD QDKGEALVLQ MWDWSFHCLS RKKPGKGEQK
 GEEEALQEGQ GRGTYRKGVG FGLLLLRLRQ RTRRHRLQQV ISLPQRASHM
 PYGKGEEGE

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          209 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:  -0.10
     Net Charge of CR: 2
     Discriminant Score:    -16.96
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -8.9
     Possible cleavage site: 34
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  10.50  threshold: -2.0
     PERIPHERAL  Likelihood = 10.50
     modified ALOM score:  -3.00
( 2) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 2
     Uncleavable? Ipos set to: 12
Discrimination of mitochondrial target seq.: 
     negative (-4.90)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.83 at (58)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region: 10.39
Chloroplast protein?  Status: negative (-3.06)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(209), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -0.53
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score: -13.10  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 140 (4) RKKP
     Found: pos: 182 (3) RRHR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:  47 (3) RK IRRANDDQGG KIRGR
Final Robbins Score (nucleus):  0.60
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.90)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.960(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>
    endoplasmic reticulum (lumen) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----