Query Information

ORIGIN plant
BEGIN
>MYSEQ
 QPEVVYRLLR RSVFGRLSGR ERRLLPQSSY STPSHRKIGQ SPNGCTSVGN
 QSKVYREIYF SSLFSYLLSR KSQKIVDFYR NPINHAFTST TLGLVEFLVA
 SVVSSCWSFI PLEFVLPRFG HHEIHHHQKH LWFVFATTDT YHIRFGSLNT
 IWKAYLIFFP TDLTLSQNSF ALRNHRRDFW TFYINKICVF GAVSNIFIVW
 VVIVKKKGLG PCKKKQKKKK KKRMKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          228 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 94 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 101   Charge diffirence(C-N):  2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:   0.49
     Net Charge of CR: 2
     Discriminant Score:    -12.24
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.92
     Possible cleavage site: 19
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -8.70  threshold: -2.0
     INTEGRAL    Likelihood = -8.70   Transmembrane  187 - 203 ( 183 - 206)
     INTEGRAL    Likelihood = -2.28   Transmembrane   94 - 110 (  94 - 110)
     PERIPHERAL  Likelihood =  6.15
     modified ALOM score:   1.84
>>> Likely a Type IIIb membrane protein (Nexo Ccyt)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 82
     YRNPIN
Discrimination of mitochondrial target seq.: 
     positive ( 1.64)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  6.71 at (52)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -1.85
     score from the 1-31 region:  6.81
Chloroplast protein?  Status: negative (-7.87)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 82)  from: 58  to: 68  Score:  3.5
Mitochondrial inner membrane?  Score:  0.80
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.368
SKL motif (signal for peroxisomal protein): 
     pos: -1(228), count: 0   
Amino Acid Composition Tendency for Peroxisome:   9.07
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score: -4.39  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 216 (5) KKKK
     Found: pos: 217 (5) KKKK
     Found: pos: 218 (5) KKKK
     Found: pos: 219 (5) KKKR
     Found: pos: 224 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos: 204 (5) KK KGLGPCKKKQ KKKKK
     Found:  pos: 205 (5) KK GLGPCKKKQK KKKKK
     Found:  pos: 212 (4) KK KQKKKKKKRM KKKKA
Final Robbins Score (nucleus):  0.48
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.08)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

     mitochondrial inner membrane --- Certainty= 0.853(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
                          nucleus --- Certainty= 0.480(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>


----- The End -----