Query Information

ORIGIN plant
BEGIN
>MYSEQ
 QHLSRRAMAK PGNRSQYILY LKFSAYQRKT TQNRRCYMLF WHPESFGIIF
 KLTTSFLLHN LRILGTEKLL EGLENGLQSS MNFVLNMFID LRFSPRRQTS
 LLTGRQGLRR RKQIKTPKHG CFVMDLGEPS EQERLLCWFH LPRSKLVMRQ
 NLILVAQITL PSTKPCFVFG SKQWESEGLY SKLIPRLFRV ILTRVARLRI
 RSLKNIWRWF EELKFPSKD

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          219 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   3
     Peak Value of UR:   1.34
     Net Charge of CR: 4
     Discriminant Score:     -5.27
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 0.92
     Possible cleavage site: 47
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   2.01  threshold: -2.0
     PERIPHERAL  Likelihood =  2.01
     modified ALOM score:  -1.30
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 64
     LRILGT
Discrimination of mitochondrial target seq.: 
     negative (-1.10)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.12 at (52)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -1.85
     score from the 1-31 region:  8.73
Chloroplast protein?  Status: negative (-5.84)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 196(219), count: 3   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   4.95
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -7.67  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 108 (5) RRRK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:  95 (4) RR QTSLLTGRQG LRRRK
Final Robbins Score (nucleus):  0.70
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.70)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.910(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.748(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----