Query Information

ORIGIN plant
BEGIN
>MYSEQ
 QEEPTRVDRL MSSSAAAGSR SAPRNRKGSC FPSLSTCSCL GLDTRFSTLP
 SHLVIYWRTL PARQWLGTLR RGLTVQKLAY ANVTESASNQ LFLKEPLSEC
 SLSSYQGSCV WESILPPSLK LPRSLDLPSK QNPCFVGLHC GILFSFPLKR
 LSYNYYYFFT EPSVIYDVLK LLTPNECMCV RLVLPDIALP PGVFCEPGIS
 GGYCASNKKD EILLCKKKKK K

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          221 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   10
     Peak Value of UR:   1.09
     Net Charge of CR: -1
     Discriminant Score:     -8.22
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 2.4
     Possible cleavage site: 21
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   1.06  threshold: -2.0
     PERIPHERAL  Likelihood =  1.06
     modified ALOM score:  -1.11
( 1) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 1
     Uncleavable? Ipos set to: 11
Discrimination of mitochondrial target seq.: 
     notclr ( 0.16)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  8.68 at (65)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.84
     score from the 1-31 region: 12.99
Chloroplast protein?  Status: notclr (-0.13)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 11)  from: 10  to: 18  Score:  3.5
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
     found at (53-55):  3.0
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-81  Score: 4423.68
Chloroplast stroma?  Score: 0.4
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 51(221), count: 1   SHL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -2.70
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score:  0.76  Status: notclr
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 215 (5) KKKK
     Found: pos: 216 (5) KKKK
     Found: pos: 217 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.70)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.700(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.434(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.400(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.300(Affirmative) < succ>


----- The End -----