Query Information

ORIGIN plant
BEGIN
>MYSEQ
 QDERPKKTNH IKEDKHQLKS SKIKLNRQRN QDPQFMKSFL IILFLATPIL
 AITVPHLTMA RHRYPYKKLT KLWFPKPNEK LKFTKLIISL FLQISRTRFL
 LRGVEFITPK ILILGLAKEI KLKYLPIEIK HLLKISNNYG NFETFKLSIH
 LKCSLGINNM CAVFIFENIC VINSKYKQIY IHIFAFMLIF SLVYFTLYFT
 LSLKCNINLK NKKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          215 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 38 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 45   Charge diffirence(C-N):  2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   1
     Peak Value of UR:  -0.15
     Net Charge of CR: 2
     Discriminant Score:    -17.61
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 6.32
     Possible cleavage site: 51
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -8.44  threshold: -2.0
     INTEGRAL    Likelihood = -8.44   Transmembrane   38 -  54 (  34 -  59)
     INTEGRAL    Likelihood = -6.00   Transmembrane  183 - 199 ( 177 - 203)
     PERIPHERAL  Likelihood =  0.32
     modified ALOM score:   1.79
>>> Likely a Type IIIb membrane protein (Nexo Ccyt)
( 1) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 1
     Uncleavable? Ipos set to: 11
Discrimination of mitochondrial target seq.: 
     negative (-7.60)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.29 at (44)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.09
     score from the 1-31 region:  5.86
Chloroplast protein?  Status: negative (-6.16)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.358
SKL motif (signal for peroxisomal protein): 
     pos: -1(215), count: 0   
Amino Acid Composition Tendency for Peroxisome:   1.96
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score:  3.42  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:   3 (4) RPKK
     Found: pos: 211 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.06)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.358(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.300(Affirmative) < succ>


----- The End -----