Query Information

ORIGIN plant
BEGIN
>MYSEQ
 PTTSKRININ SSLQRIKLNR QRNQDPQFMK SFLIILFLAT TYTCHNRTSS
 DIINGTISIK TLNKTLVSKT KETKILDQTY YIPFLTNLED EISVKGGRIY
 NTNPNFGVSK RNQTKVSPNR NLKITSLKMN LWKFINIIVY TFKMMFSWNK
 YVCGIHIKHL RNQLIKIIQT NIYTHICIYV DLFVGVFYPV FYPKFKMHKL
 EVEIKKKKKN S

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          211 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 28 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 35   Charge diffirence(C-N): -2.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   8
     Peak Value of UR:   0.09
     Net Charge of CR: 2
     Discriminant Score:    -13.82
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 2.66
     Possible cleavage site: 43
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.23  threshold: -2.0
     INTEGRAL    Likelihood = -2.23   Transmembrane  176 - 192 ( 175 - 192)
     PERIPHERAL  Likelihood = -0.00
     modified ALOM score:   0.55
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 175 (175 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 49
     NRTSSD
Discrimination of mitochondrial target seq.: 
     positive ( 1.79)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.15 at (52)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.99
     score from the 1-31 region:  8.31
Chloroplast protein?  Status: negative (-1.29)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 82%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 49)  from: 32  to: 39  Score:  8.0
Mitochondrial inner membrane?  Score:  0.33
Mitochondrial intermemb.space?  Score:  0.09
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.109
SKL motif (signal for peroxisomal protein): 
     pos: -1(211), count: 0   
Amino Acid Composition Tendency for Peroxisome:   0.25
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.100
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -2.27  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 204 (5) KKKK
     Found: pos: 205 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.21)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

     mitochondrial inner membrane --- Certainty= 0.515(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.440(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.344(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.279(Affirmative) < succ>


----- The End -----