Query Information

ORIGIN plant
BEGIN
>MYSEQ
 PRKAKEDQPH QRGTSTQVFK DQVESPAESR STVLDEVFPY YTLPCHTYTC
 HNRTSSDIIN GTISIKTLNK TLVSKTKETK ILDQTYYIPF LTNLEDEISV
 KGGRIYNTNP NFGVSKRNQT KVSPNRNLKI TSLKMNLWKF INIIVYTFKM
 MFSWNKYVCG IHIKHLRNQL IKIIQTNIYT HICIYVDLFV GVFYPVFYPK
 FKMHKLEVKL KKKK

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          214 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 182 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 189   Charge diffirence(C-N):  8.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   1
     Peak Value of UR:  -0.37
     Net Charge of CR: 2
     Discriminant Score:    -19.18
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -6.24
     Possible cleavage site: 49
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.23  threshold: -2.0
     INTEGRAL    Likelihood = -2.23   Transmembrane  182 - 198 ( 181 - 198)
     PERIPHERAL  Likelihood =  4.35
     modified ALOM score:   0.55
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 199 to 214 (17 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 4
     PRKAKE
Discrimination of mitochondrial target seq.: 
     negative (-4.96)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.15 at (58)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.00
     score from the 1-31 region:  7.90
Chloroplast protein?  Status: negative (-3.69)

*** Reasoning Step: 2

> Relative position of the end of the tail: 92%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.109
SKL motif (signal for peroxisomal protein): 
     pos: -1(214), count: 0   
Amino Acid Composition Tendency for Peroxisome:   1.05
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.207
Amino acid composition tendency for vacuolar proteins
     Score: -2.85  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 210 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.15)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.207(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.200(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.109(Affirmative) < succ>


----- The End -----