Query Information

ORIGIN plant
BEGIN
>MYSEQ
 PKFIFKNSKN YKKITKNICK STYFTNNYYI FLIIILLVSL FKMNYTSRKK
 SNACKENDEN KLNTKINLLP LIYLNLLFLM AFMVLQTYPS KESRPRDETS
 ENSGNSALSS SSLSQVASSS ELHTLHMLMT LLRVILRDVS RILIGYSEVK
 SSLTFSSSMG NCSSGTLKHF LSCDTNTSCT SDNLSGSSNW ATSPFLSKIL
 NGPIRGDNFP LTLNLFIPLI GETLRTKSPT STTIL

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          235 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 30 at i=2
MTOP: membrane topology (Hartmann et al.)
     I(middle): 37   Charge diffirence(C-N): -2.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:   0.30
     Net Charge of CR: 3
     Discriminant Score:    -13.49
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -4.52
     Possible cleavage site: 46
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 2 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 2  value:  -7.01  threshold: -2.0
     INTEGRAL    Likelihood = -7.01   Transmembrane   68 -  84 (  65 -  88)
     INTEGRAL    Likelihood = -4.14   Transmembrane   30 -  46 (  24 -  47)
     PERIPHERAL  Likelihood =  1.38
     modified ALOM score:   1.50
>>> Likely a Type IIIa membrane protein (Ncyt Cexo)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 50
     SRKKSN
Discrimination of mitochondrial target seq.: 
     negative (-2.79)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.63 at (57)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  5.45
Chloroplast protein?  Status: negative (-5.62)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.300
SKL motif (signal for peroxisomal protein): 
     pos: -1(235), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -2.95
Peroxisomal proteins?   Status: negative
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score:  4.60  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.300(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.300(Affirmative) < succ>


----- The End -----