Query Information

ORIGIN plant
BEGIN
>MYSEQ
 PEIHFELKLE NYQKYLMKYL FLDLLYFLNY YLITSISFNE LILNISEIKC
 MQRKRKIIKH NKFITLINII SKFIILIDGF YGVTNLPLLK RISSSRLRNF
 RELREFCSEF IFSFPSGFIF RMMTPLNFAH VNDFTPCNSA RCFQNSDWVL
 RISQIFINIQ FFHGLFFGYS ALLELHVKYV MHISIGLLVG YFAFSFQNLE
 WANIARFSFN LKPFHSSHRN FKVDKVSYFN NNSL

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          234 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 63 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 70   Charge diffirence(C-N): -7.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   3
     Peak Value of UR:   2.00
     Net Charge of CR: -1
     Discriminant Score:     -3.98
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.73
     Possible cleavage site: 37
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -2.66  threshold: -2.0
     INTEGRAL    Likelihood = -2.66   Transmembrane   63 -  79 (  61 -  80)
     PERIPHERAL  Likelihood = -1.75
     modified ALOM score:   0.63
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 62 (62 Residues)
( 1) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 1
     Uncleavable? Ipos set to: 11
Discrimination of mitochondrial target seq.: 
     negative (-6.22)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.82 at (34)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.95
     score from the 1-31 region:  1.48
Chloroplast protein?  Status: negative (-9.25)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 26%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial inner membrane?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.126
SKL motif (signal for peroxisomal protein): 
     pos: 95(234), count: 1   SRL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   8.98
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.640
Checking the consensus for Golgi
Checking the consensus for Golgi
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score:  4.44  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  52 (5) RKRK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.15)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.790(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.748(Affirmative) < succ>
                       Golgi body --- Certainty= 0.300(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.200(Affirmative) < succ>


----- The End -----