Query Information

ORIGIN plant
BEGIN
>MYSEQ
 NPSKETSTLR TTSRYFSSFG SLALQLLSIP ETTWESVLSI ALLIPIAFSF
 RRPRSRASYS AMLFVQLKSS FAAQVLTLEG ETNTAAAPAP KVPQDPSQNT
 IHASASLFAS SSAPGVQSAM KSANAWDIED LTYSIQNSLS SAAHFPILPV
 ASRFLIISFR GCEEGTIMLA KCRSFLDAIK TAYNTFSNSV FFFDMLRTSD
 TKYTGTCFRA WPFKLSWTSA 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          220 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 34 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 41   Charge diffirence(C-N):  6.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   3
     Peak Value of UR:   1.54
     Net Charge of CR: 1
     Discriminant Score:     -5.87
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 3.45
     Possible cleavage site: 49
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -1.59  threshold: -2.0
     PERIPHERAL  Likelihood = -1.59
     modified ALOM score:  -0.58
 count: 1  itm.site: 16)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 24
     (count:1)
     (itm.site: 16)
     SRYFSS
Discrimination of mitochondrial target seq.: 
     notclr ( 0.69)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  9.85 at (43)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.10
     score from the 1-31 region: 12.05
Chloroplast protein?  Status: notclr ( 1.19)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 24)  from: 37  to: 50  Score:  9.0
Mitochondrial matrix?  Score:  0.36
Mitochondrial intermemb.space?  Score:  0.30
Checking apolar signal for intrachloroplastic sorting
     found at (40-50):  7.5
Howe: Checking the consensus for intrachloropl.sorting
     Precursor 1-89  Score: 19988.48
Chloroplast stroma?  Score: 0.4
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(220), count: 0   
Amino Acid Composition Tendency for Peroxisome:   5.21
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -1.73  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos:  50 (4) RRPR
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.640(Affirmative) < succ>
               chloroplast stroma --- Certainty= 0.532(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.468(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.418(Affirmative) < succ>


----- The End -----