Query Information

ORIGIN plant
BEGIN
>MYSEQ
 NKIRSYGSTR WTDHDVVRLM LRSFTVLDPH LVNNIRENPR YTKMTPEEVL
 GKFVSGRMMI KEARYVDDAL NGPINEPQPL ARKAMRSKEA LPSKVAQVEA
 AVLNNEEMAL IIKRFKTALK GRKGQPSKTK TKEKRSCFKC GKIGHFIANC
 PENESDQEKG NKREKKKHYK KAKGEAHIGK EWDLDCSSSD SDNEGLAATA
 FNKSSLFPNE RHTCLMAREK KVS

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          223 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   2
     Peak Value of UR:   1.31
     Net Charge of CR: 3
     Discriminant Score:     -6.41
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -4.38
     Possible cleavage site: 29
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   4.77  threshold: -2.0
     PERIPHERAL  Likelihood =  4.77
     modified ALOM score:  -1.85
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 12
     TRWTDH
Discrimination of mitochondrial target seq.: 
     notclr ( 0.12)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.41 at (69)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.11
     score from the 1-31 region:  4.08
Chloroplast protein?  Status: negative (-8.45)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 12)  from: 19  to: 21  Score:  2.5
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(223), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -4.90
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score:  1.75  Status: notclr
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  20.2% ( 0.31)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 164 (3) KKKH
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.80
Nuclear Signal   Status: notclr ( 0.60)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.724(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.432(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.112(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.112(Affirmative) < succ>


----- The End -----