Query Information

ORIGIN plant
BEGIN
>MYSEQ
 NGGEEGGGYT KSTTNRRLQA RQGNGARHQE FPPNPQTKEG ERLQVPLQEG
 LLQVWARSFY CMSYIQPRRQ EGETKGKEEV LQEEGRRCPC LSRMGLRREL
 KRLLRRRGRR QHRRHQGTPL PQRRPQVPHG KGRQKEGIIL HIILQCGRFA
 FPFCQPHSSK RKIEIGCYSK RRPFGFPRGL S

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          181 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 138 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 145   Charge diffirence(C-N):  1.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   4
     Peak Value of UR:  -1.28
     Net Charge of CR: -1
     Discriminant Score:    -26.57
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.33
     Possible cleavage site: 56
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  -0.37  threshold: -2.0
     PERIPHERAL  Likelihood = -0.37
     modified ALOM score:  -0.83
( 3) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 3
     Uncleavable? Ipos set to: 13
Discrimination of mitochondrial target seq.: 
     negative (-5.66)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.74 at (43)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.01
     score from the 1-31 region:  6.00
Chloroplast protein?  Status: negative (-4.69)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(181), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -0.97
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score: -13.62  Status: negative
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  21.5% ( 0.38)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 169 (4) KRRP
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
     Found:  pos:  85 (3) RR CPCLSRMGLR RELKR
     Found:  pos:  96 (3) RR ELKRLLRRRG RRQHR
Final Robbins Score (nucleus):  0.80
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.90)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.988(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>
 endoplasmic reticulum (membrane) --- Certainty= 0.000(Not Clear) < succ>


----- The End -----