Query Information

ORIGIN plant
BEGIN
>MYSEQ
 MPCIGSNGLY TVSSLCKFPC VSTALLPFHD SSCLNIWRWV RPSLMAMEEV
 MMPKLLQSLG SRLAITASRS CKPVARNEFR RLHRILMCKK MHWLRRARDE
 YLLALGSGTK TVCNAGTTKV ATLTIKAYGT VSCYGACLRC LEFPIHAARQ
 WLHVKMGTVF QGVGKIPIIT IIQTTTTCTR PTRIINQTLV WILGLKTQNY
 IGYRVTCLGA ITRN

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          214 Residues
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   15
     Peak Value of UR:   1.34
     Net Charge of CR: 0
     Discriminant Score:     -4.28
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -2.2
     Possible cleavage site: 33
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   1.54  threshold: -2.0
     PERIPHERAL  Likelihood =  1.54
     modified ALOM score:  -1.21
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 43
     VRPSLM
Discrimination of mitochondrial target seq.: 
     negative (-1.82)
Hydrophobic moment analysis for chloroplast proteins 
     Hmax:  7.92 at (61)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  3.88
     score from the 1-31 region:  7.17
Chloroplast protein?  Status: negative (-1.45)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
Mitochondrial matrix?  Score:  0.10
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: 61(214), count: 1   SRL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:  -0.73
Peroxisomal proteins?   Status: notclr
Amino acid composition tendency for vacuolar proteins
     Score: -6.39  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..
Cytoplasmic protein?   Score2: 0.450

----- Final Results -----

                        cytoplasm --- Certainty= 0.450(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.300(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.100(Affirmative) < succ>
   chloroplast thylakoid membrane --- Certainty= 0.100(Affirmative) < succ>


----- The End -----