Query Information

ORIGIN plant
BEGIN
>MYSEQ
 LTFFSLAIRH VRSLGKRDDL LKAVAASPSL SESEEEQSKS HSFPICASPL
 AFLCFFFSLL FPFSWSLSFS GQLAMKPILP HLKHERFSFV LVLLGCPLRP
 LSAVLKRLMM RAISSLLSTA ASTCATLLGS ASLLLIALRA RGCGSLIGPF
 KASSTYLASL IIIRPLTNFP STSSGVIFVY LGFSRILFTR GSRTVNDLSI
 RRTTSSVHRV LPLLILL

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          217 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 3
     Position of the most N-terminal TMS: 45 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 52   Charge diffirence(C-N):  5.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   1
     Peak Value of UR:   1.56
     Net Charge of CR: 2
     Discriminant Score:     -5.64
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 1.44
     Possible cleavage site: 13
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 3  value:  -6.48  threshold:  0.5
     INTEGRAL    Likelihood = -6.48   Transmembrane   45 -  61 (  42 -  69)
     INTEGRAL    Likelihood = -3.56   Transmembrane   89 - 105 (  87 - 106)
     INTEGRAL    Likelihood = -2.18   Transmembrane  122 - 138 ( 120 - 140)
     PERIPHERAL  Likelihood =  1.70
     modified ALOM score:   1.90
>>> Likely a Type IIIb membrane protein (Nexo Ccyt)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 14
     VRSLGK
Discrimination of mitochondrial target seq.: 
     positive ( 1.58)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.01 at (59)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  0.03
     score from the 1-31 region:  9.08
Chloroplast protein?  Status: negative (-2.52)

*** Reasoning Step: 2

Type IIIa or IIIb is favored for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 14)  from: 43  to: 61  Score: 11.0
>>> Seems to have an intramitochondrial signal
Mitochondrial inner membrane?  Score:  0.34
Mitochondrial intermemb.space?  Score:  0.22
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.379
SKL motif (signal for peroxisomal protein): 
     pos: -1(217), count: 0   
Amino Acid Composition Tendency for Peroxisome:   3.46
Peroxisomal proteins?   Status: notclr
Type III proteins may be localized at Golgi
Amino acid composition tendency for vacuolar proteins
     Score:  3.15  Status: positive
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
Nuclear Signal   Status: negative ( 0.00)
Check the Number of TMSs for typeIII (plasma memb.)
Checking N-myristoylation..

----- Final Results -----

                  plasma membrane --- Certainty= 0.600(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.511(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.425(Affirmative) < succ>
                       Golgi body --- Certainty= 0.400(Affirmative) < succ>


----- The End -----