Query Information

ORIGIN plant
BEGIN
>MYSEQ
 LTCQGRFNRL RGRLNRPQAE NFAQRLVLSS HYIYYLLPLS PKARFELHFP
 KKHLPLFFTH FLPLPFQIFG EKSLSELESC GFVLHLISLA LLLHSSFGTT
 SSSLWIHYSW SFLLDDVFLV SLQILWKTTR KFVLPARLSK DCVSLTFVVG
 KGRIRVERDP ALCGRLNEEV GHLGGVTEPR DKKKKKKKKK 

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          190 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 1
     Position of the most N-terminal TMS: 77 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 84   Charge diffirence(C-N):  2.5
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   1
     Peak Value of UR:   0.95
     Net Charge of CR: 3
     Discriminant Score:     -9.25
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -3.84
     Possible cleavage site: 45
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -3.61  threshold: -2.0
     INTEGRAL    Likelihood = -3.61   Transmembrane   77 -  93 (  77 -  93)
     PERIPHERAL  Likelihood =  1.70
     modified ALOM score:   0.82
>>> Seems to be a Type Ib (Nexo Ccyt) membrane protein
     The cytoplasmic tail is from 94 to 190 (98 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 46
     ARFELH
Discrimination of mitochondrial target seq.: 
     notclr ( 0.31)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 10.08 at (61)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.08
     score from the 1-31 region:  7.47
Chloroplast protein?  Status: negative (-3.35)

*** Reasoning Step: 2

> Relative position of the end of the tail: 49%
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 46)  from: 26  to: 28  Score:  3.0
Mitochondrial inner membrane?  Score:  0.33
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.164
SKL motif (signal for peroxisomal protein): 
     pos: 136(190), count: 1   ARL
     SKL score (peroxisome):  0.3
Amino Acid Composition Tendency for Peroxisome:   6.59
Peroxisomal proteins?   Status: positive
     AAC score (peroxisome): 0.640
Amino acid composition tendency for vacuolar proteins
     Score: -0.64  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 181 (5) KKKK
     Found: pos: 182 (5) KKKK
     Found: pos: 183 (5) KKKK
     Found: pos: 184 (5) KKKK
     Found: pos: 185 (5) KKKK
     Found: pos: 186 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.65
Nuclear Signal   Status: notclr ( 0.35)
Check cytoplasmic tail for typeIb (plasma memb.)
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

           microbody (peroxisome) --- Certainty= 0.748(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.700(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.417(Affirmative) < succ>
                          nucleus --- Certainty= 0.350(Affirmative) < succ>


----- The End -----