Query Information

ORIGIN plant
BEGIN
>MYSEQ
 LSPQRRERTL QMSGTDTTSS NVRRPQEETR DQSMSKTHTG RACFDRVSGA
 QLNVDIMNAR IRIPGRETRA STLLPCADRR IQGPGAQLRW RGRHSKCVCG
 SDRLILRTPS PRNQEKNKCV SFQISETEKR DLFRSAPHYA PLQQMRVFGG
 TGEPRELVRC VGQRARSTRS YAVEGKEPKS TPFFLFKKKK K

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          191 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   7
     Peak Value of UR:  -0.49
     Net Charge of CR: 2
     Discriminant Score:    -18.14
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -5.83
     Possible cleavage site: 50
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:  10.56  threshold: -2.0
     PERIPHERAL  Likelihood = 10.56
     modified ALOM score:  -3.01
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 7
     QRRERT
Discrimination of mitochondrial target seq.: 
     notclr ( 0.55)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 13.44 at (45)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -1.76
     score from the 1-31 region:  7.79
Chloroplast protein?  Status: negative (-4.92)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 7)  from: 42  to: 44  Score:  2.5
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
SKL motif (signal for peroxisomal protein): 
     pos: -1(191), count: 0   
Amino Acid Composition Tendency for Peroxisome:  -3.20
Peroxisomal proteins?   Status: negative
Amino acid composition tendency for vacuolar proteins
     Score: -11.81  Status: negative
Checking the amount of Basic Residues (nucleus)
     Highly rich in K&R:  20.9% ( 0.35)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 186 (5) KKKK
     Found: pos: 187 (5) KKKK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.90
Nuclear Signal   Status: positive ( 0.90)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.935(Affirmative) < succ>
       mitochondrial matrix space --- Certainty= 0.459(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.155(Affirmative) < succ>
mitochondrial intermembrane space --- Certainty= 0.155(Affirmative) < succ>


----- The End -----