Query Information

ORIGIN plant
BEGIN
>MYSEQ
 LRTILLSNFL SVPKPNLGTA LLQHSTTESI LEMMTCPQKL VSFSAFVKLA
 LLVSSLSLST SVFLVQISVE RKRFEVSRAL SFGILHLGTT LDFLLADSSA
 NSCIACSALF TLLGGVEQPY TSLPPLNHLE KRKRKTSQDG FPRSVQSGLY
 FFKLAHILPY RSNLMSFSFI NWVTDLDAQK PILDLTNQGV PIRMRVTDHI
 TPVPLTSLQH NKRFFLQCVS QRFFFSF

Result Information

PSORT --- Prediction of Protein Localization Sites
                                            version 6.4(WWW)
MYSEQ          227 Residues
*** Warning: The 1st amino acid is not methionine
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 2
     Position of the most N-terminal TMS: 49 at i=1
MTOP: membrane topology (Hartmann et al.)
     I(middle): 56   Charge diffirence(C-N):  0.0
McG: Examining signal sequence (McGeoch)
     Length  of  UR:   11
     Peak Value of UR:   1.71
     Net Charge of CR: 1
     Discriminant Score:     -2.21
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): 5.38
     Possible cleavage site: 61
>>> Seems to have no N-terminal signal seq.
Amino Acid Composition of Predicted Mature Form:
   calculated from 1
ALOM new cnt: 1 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 1  value:  -4.46  threshold: -2.0
     INTEGRAL    Likelihood = -4.46   Transmembrane   49 -  65 (  46 -  67)
     PERIPHERAL  Likelihood =  0.10
     modified ALOM score:   0.99
>>> Seems to be a Type II (Ncyt Cexo) membrane protein
     The cytoplasmic tail is from 1 to 48 (48 Residues)
Gavel: Examining the boundary of mitochondrial targeting seq.
      motif at: 4
     LRTILL
Discrimination of mitochondrial target seq.: 
     notclr ( 0.12)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins 
     Hmax: 11.93 at (66)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region:  1.96
     score from the 1-31 region:  8.15
Chloroplast protein?  Status: negative (-1.26)

*** Reasoning Step: 2

Relative position of the cytoplasmic tail: 21%
     Larger value (>30%) is favared for ER memb. proteins
KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 4)  from: 40  to: 65  Score:  8.0
Mitochondrial inner membrane?  Score:  0.40
Mitochondrial intermemb.space?  Score:  0.09
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.198
SKL motif (signal for peroxisomal protein): 
     pos: -1(227), count: 0   
Amino Acid Composition Tendency for Peroxisome:   3.67
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.556
Checking the consensus for Golgi
Checking the consensus for Golgi
(S/T)X(E/Q)(R/K) motif found at 68
Checking the cytoplasmic tail of type II (Golgi)
Amino acid composition tendency for vacuolar proteins
     Score: -3.34  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
     Found: pos: 130 (5) KRKR
     Found: pos: 131 (5) RKRK
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins & Dingwall consensus (nucleus)
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.60
Nuclear Signal   Status: notclr ( 0.30)
Check mitochondrial signal for typeII (plasma memb.)
Type II is favored for plasma memb. proteins
Checking the NPXY motif..
Checking the YXRF motif..
Checking N-myristoylation..

----- Final Results -----

                       Golgi body --- Certainty= 0.900(Affirmative) < succ>
                  plasma membrane --- Certainty= 0.650(Affirmative) < succ>
           microbody (peroxisome) --- Certainty= 0.556(Affirmative) < succ>
     mitochondrial inner membrane --- Certainty= 0.464(Affirmative) < succ>


----- The End -----